| # | Title | Artist | Rating | Length |
|---|
| 1 | Crazy Blues | Mamie Smith and Her Jazz Hounds | | 3:29 |
| 2 | The St. Louis Blues | Bessie Smith | | 3:10 |
| 3 | Match Box Blues | Blind Lemon Jefferson | | 2:58 |
| 4 | Jim Jackson's Kansas City Blues, Pt. 1 | Jim Jackson | | 3:17 |
| 5 | Pine Top's Boogie Woogie | Pine Top Smith | | 3:12 |
| 6 | See See Rider Blues | 'Ma' Rainey & Her Georgia Jazz Band | | 3:19 |
| 7 | Statesboro Blues | Blind Willie McTell | | 2:38 |
| 8 | How Long–How Long Blues, Pt. 1 | Leroy Carr | | 3:05 |
| 9 | Big Road Blues | Tommy Johnson | | 3:21 |
| 10 | Troube in Mind | Bertha “Chippie” Hill | | 2:52 |
| 11 | Mother's Children Have a Hard Time | Blind Willie Johnson | | 3:24 |
| 12 | Divin' Duck Blues | Sleepy John Estes | | 3:16 |
| 13 | Black Snake Blues | Victoria Spivey | | 3:09 |
| 14 | Diddie Wa Diddie | Blind Blake | | 2:59 |
| 15 | It's Tight Like That | Tampa Red and Georgia Tom | | 3:04 |
| 16 | I'm a Mighty Tight Woman | Sippie Wallace | | 2:53 |
| 17 | Candy Man Blues | Mississippi John Hurt | | 2:49 |
| 18 | Pan-American Blues | DeFord Bailey | | 3:01 |
| 19 | Pony Blues | Charley Patton | | 3:01 |
| 20 | Sitting on Top of the World | Mississippi Sheiks | 3 | 3:14 |