| # | Title | Artist | Rating | Length |
|---|
| 1 | Sun Brimmers Blues | Memphis Jug Band | | 3:26 |
| 2 | Need More Blues | Robert Cocksey | | 3:14 |
| 3 | Up Country Blues | De Ford Bailey | | 3:15 |
| 4 | Man Trouble Blues | Jaybird Coleman | 5 | 3:04 |
| 5 | Greenville Strut | Daddy Stovepipe & Mississippi Sarah | | 3:30 |
| 6 | Chickasaw Special | Noah Lewis | 5 | 3:16 |
| 7 | The Old Folk Started | Minnie Wallace | 5 | 2:43 |
| 8 | Touch Me Light Mama | George "Bullet" Williams | 5 | 2:46 |
| 9 | Fourth Avenue Blues | Slim Barton & Eddie Mapp | | 2:48 |
| 10 | El Watson's Fox Chase | El Watson | | 2:53 |
| 11 | Medley of Blues | Freeman Stowers | 5 | 2:54 |
| 12 | Beale Street Breakdown | Jed Davenport & His Jug Band | | 2:57 |
| 13 | Police and High Sheriff Come Ridin' Down | Ollis Martin | | 3:01 |
| 14 | Bumble Bee Blues | Memphis Minnie | | 2:52 |
| 15 | Mean Low Blues | Blues Birdhead | | 3:22 |
| 16 | Mississippi Swamp Moon | Alfred Lewis | 5 | 3:23 |
| 17 | Tampa Strut | Georgia Browns | | 2:56 |
| 18 | Harmonica Stomp | Jazz Gillum | | 2:48 |
| 19 | I'm a Stranger Here | Blind Boy Fuller | | 2:54 |
| 20 | Blues in the Rain | Eddie Kelly's Washboard Band | | 2:29 |
| 21 | You Can Lead Me | Sonny Boy Williamson | | 3:08 |
| 22 | Slick Capers Blues | Little Buddy Doyle | | 2:37 |
| 23 | Blowing the Blues | Sonny Terry | | 2:53 |
| 24 | We Got to Get Together | Frank Edwards | | 2:37 |
| 25 | Tampa Blues | Skoodle Dum Doo & Sheffield | | 2:57 |