| # | Title | Artist | Rating | Length |
|---|
| 1 | Po Lazarus (from “O Brother, Where Art Thou?” soundtrack) | James Carter and the Prisoners | 3.4 | 4:33 |
| 2 | Big Rock Candy Mountain | Harry McClintock | 3 | 2:19 |
| 3 | You Are My Sunshine | Norman Blake | 3.5 | 4:24 |
| 4 | Down to the River to Pray- recording engineer and mixer:
- Mike Piersante
- assistant engineer:
- Matt Andrews and Rail Rogut
- producer:
- T-Bone Burnett
- assistant mixer:
- Ok Hee Kim
- additional vocals:
- Norman Blake , Dub Cornett, Pat Enright, Porter McClister, Tim O’Brien , Maura O’Connell, Sam Phillips , David Rawlings and Gillian Welch
- choir vocals:
- The First Baptist Choir of White House, Tennessee
- lead vocals:
- Alison Krauss
- phonographic copyright (℗) by:
- Mercury Records (in 2000)
- recorded at:
- Sound Emporium Studios in Nashville, Tennessee, United States
- recording of:
- The Good Old Way
- writer:
- [traditional]
| Alison Krauss | 4 | 2:57 |
| 5 | I Am a Man of Constant Sorrow (radio station version) | The Soggy Bottom Boys feat. Dan Tyminski | 4.2 | 3:10 |
| 6 | Hard Time Killing Floor Blues | Chris Thomas King | 4 | 2:44 |
| 7 | I Am a Man of Constant Sorrow (instrumental) | Norman Blake | 3.5 | 4:29 |
| 8 | Keep on the Sunny Side | The Whites | 3.35 | 3:35 |
| 9 | I’ll Fly Away | Gillian Welch & Alison Krauss | 4 | 3:58 |
| 10 | Didn’t Leave Nobody but the Baby | Emmylou Harris, Alison Krauss & Gillian Welch | 3.8 | 1:59 |
| 11 | In the Highways | The Peasall Sisters | 3 | 1:37 |
| 12 | I Am Weary (Let Me Rest) | The Cox Family | 3.65 | 3:16 |
| 13 | I Am a Man of Constant Sorrow (instrumental) | John Hartford | 3 | 2:36 |
| 14 | O Death | Ralph Stanley | 3.5 | 3:21 |
| 15 | In the Jailhouse Now | The Soggy Bottom Boys feat. Tim Blake Nelson | 4 | 3:37 |
| 16 | I Am a Man of Constant Sorrow | The Soggy Bottom Boys | 3.75 | 4:17 |
| 17 | Indian War Whoop | John Hartford | 3 | 1:32 |
| 18 | Lonesome Valley | Fairfield Four | 3.35 | 4:09 |
| 19 | Angel Band | The Stanley Brothers & The Clinch Mountain Boys | 3.35 | 2:21 |