| # | Title | Artist | Rating | Length |
|---|
| 1 | Don’t Get Above Your Rising | Lester Flatt & Earl Scruggs | | 2:42 |
| 2 | Man of Constant Sorrow | The Stanley Brothers | | 2:57 |
| 3 | Great Speckled Bird | Roy Acuff | | 2:54 |
| 4 | Orange Blossom Special | Rouse Brothers | | 2:57 |
| 5 | Y’all Come | Bill Monroe and the Bluegrass Boys | | 2:10 |
| 6 | Are You From Dixie? | The Blue Sky Boys | | 2:43 |
| 7 | Cotton Eyed Joe | Fiddlin’ John Carson | | 3:04 |
| 8 | Foggy Mountain Breakdown | Lester Flatt & Earl Scruggs | | 2:40 |
| 9 | Going Back to the Blue Ridge Mountains | The Delmore Brothers | | 2:48 |
| 10 | Air Mail Special | Jim and Jesse | | 2:06 |
| 11 | Blue Grass Breakdown | Bill Monroe and the Bluegrass Boys | | 2:17 |
| 12 | Little Glass of Wine | The Stanley Brothers | | 2:56 |
| 13 | Earl’s Breakdown | Lester Flatt & Earl Scruggs | | 2:59 |
| 14 | Pretty Polly | The Coon Creek Girls | | 2:48 |
| 15 | Heart’s Hall of Fame | Bailey Brothers | | 3:00 |
| 16 | Will the Circle Be Unbroken? | The Monroe Brothers | | 2:28 |
| 17 | Twin Banjo Special | Joe Maphis | | 2:12 |
| 18 | Story of the Knoxville Girl | The Blue Sky Boys | | 3:35 |
| 19 | Blue Eyed Darling | Jimmy Martin & the Sunny Mountain Boys | | 2:21 |
| 20 | I’m Gone Long Gone | Don Reno & The Tennessee Cut-Ups | | 2:19 |
| 21 | Sally Goodin | Ernest V. Stoneman and His Dixie Mountaineers | | 3:39 |
| 22 | Uncle Pen | Bill Monroe and the Bluegrass Boys | | 2:44 |
| 23 | Cannon Ball Blues | The Carter Family | | 2:54 |
| 24 | Don’t Let Your Deal Go Down | Charlie Poole & The North Carolina Ramblers | | 2:51 |
| 25 | When the Train Comes Along | Uncle Dave Macon | | 3:04 |
| 26 | Foggy Mountain Special | Lester Flatt & Earl Scruggs | | 1:59 |
| 27 | Save It, Save It | Jimmy Martin & the Sunny Mountain Boys | | 1:59 |